Journal: International Journal of Molecular Sciences

Article Title: Evaluation of the Antioxidant Properties and Bioactivity of Koroneiki and Athinolia Olive Varieties Using In Vitro Cell-Free and Cell-Based Assays

doi: 10.3390/ijms26020743

Figure Lengend Snippet: Heat map of the antioxidant score, corresponding to the antioxidant potential of Greek olive samples for the assays DPPH•, ABTS •+ , Superoxide radical (O 2 •− ), Hydroxyl radical (OH•), Reducing power (RP), and Peroxyl radical (ROO•)-induced plasmid DNA relaxation assay. The color scale from gray to white represents the Antioxidant score values of the normalized antioxidant capacity data, from low to high, respectively, with 0 representing the lowest antioxidant score and 1 the highest.

Article Snippet: In brief, plasmid DNA (3.2 μg/mL, pBluescript SK+) (Fermentas, Waltham, MA, USA), phosphate-buffered saline (PBS), 2,2′ -azobis (2-amidinopropane hydrochloride) (AAPH) solution (95 mM in PBS), and the aqueous phase diluted at various concentrations were added to test tubes, to a final volume of 10 μL.

Techniques: Plasmid Preparation

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of the Antioxidant Properties and Bioactivity of Koroneiki and Athinolia Olive Varieties Using In Vitro Cell-Free and Cell-Based Assays

doi: 10.3390/ijms26020743

Figure Lengend Snippet: Heat map of the antioxidant score, corresponding to the antioxidant potential of Athinolia samples for the assays DPPH•, ABTS •+ , Superoxide radical (O 2 •− ), Hydroxyl radical (OH•), Reducing power (RP), and Peroxyl radical (ROO•)-induced plasmid DNA relaxation assay. (Grove 2: rainfed, 630 m, Grove 3: rainfed, 270 m, Grove 4: irrigated, 270 m). The color scale from gray to white represents the Antioxidant score values of the normalized antioxidant capacity data, from low to high, respectively, with 0 representing the lowest antioxidant score and 1 the highest.

Article Snippet: In brief, plasmid DNA (3.2 μg/mL, pBluescript SK+) (Fermentas, Waltham, MA, USA), phosphate-buffered saline (PBS), 2,2′ -azobis (2-amidinopropane hydrochloride) (AAPH) solution (95 mM in PBS), and the aqueous phase diluted at various concentrations were added to test tubes, to a final volume of 10 μL.

Techniques: Plasmid Preparation

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of the Antioxidant Properties and Bioactivity of Koroneiki and Athinolia Olive Varieties Using In Vitro Cell-Free and Cell-Based Assays

doi: 10.3390/ijms26020743

Figure Lengend Snippet: Heat map of the antioxidant score, corresponding to the antioxidant potential of Athinolia samples for the assays DPPH•, ABTS •+ , Superoxide radical (O 2 •− ), Hydroxyl radical (OH•), Reducing power (RP), and Peroxyl radical (ROO•)-induced plasmid DNA relaxation assay, with respect to the altitude (Grove 2: 630 m, Grove 3: 270 m). The color scale from gray to white represents the Antioxidant score values of the normalized antioxidant capacity data, from low to high, respectively, with 0 representing the lowest antioxidant score and 1 the highest.

Article Snippet: In brief, plasmid DNA (3.2 μg/mL, pBluescript SK+) (Fermentas, Waltham, MA, USA), phosphate-buffered saline (PBS), 2,2′ -azobis (2-amidinopropane hydrochloride) (AAPH) solution (95 mM in PBS), and the aqueous phase diluted at various concentrations were added to test tubes, to a final volume of 10 μL.

Techniques: Plasmid Preparation

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of the Antioxidant Properties and Bioactivity of Koroneiki and Athinolia Olive Varieties Using In Vitro Cell-Free and Cell-Based Assays

doi: 10.3390/ijms26020743

Figure Lengend Snippet: Heat map of the antioxidant score, corresponding to the antioxidant potential of Athinolia samples for the assays DPPH•, ABTS •+ , Superoxide radical (O 2 •− ), Hydroxyl radical (OH•), Reducing power (RP), and Peroxyl radical (ROO•)-induced plasmid DNA relaxation assay, with respect to the irrigation regime (Grove 3: rainfed, Grove 4: irrigated). The color scale from gray to white represents the Antioxidant score values of the normalized antioxidant capacity data, from low to high, respectively, with 0 representing the lowest antioxidant score and 1 the highest.

Article Snippet: In brief, plasmid DNA (3.2 μg/mL, pBluescript SK+) (Fermentas, Waltham, MA, USA), phosphate-buffered saline (PBS), 2,2′ -azobis (2-amidinopropane hydrochloride) (AAPH) solution (95 mM in PBS), and the aqueous phase diluted at various concentrations were added to test tubes, to a final volume of 10 μL.

Techniques: Plasmid Preparation

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of the Antioxidant Properties and Bioactivity of Koroneiki and Athinolia Olive Varieties Using In Vitro Cell-Free and Cell-Based Assays

doi: 10.3390/ijms26020743

Figure Lengend Snippet: Heat map of the antioxidant score, corresponding to the antioxidant potential of Koroneiki samples for the assays DPPH•, ABTS •+ , Superoxide radical (O 2 •− ), Hydroxyl radical (OH•), Reducing power (RP), and Peroxyl radical (ROO•)-induced plasmid DNA relaxation assay (Grove 1: rainfed, 580 m, Grove 5: irrigated, 152 m). The color scale from gray to white represents the Antioxidant score values of the normalized antioxidant capacity data, from low to high, respectively, with 0 representing the lowest antioxidant score and 1 the highest.

Article Snippet: In brief, plasmid DNA (3.2 μg/mL, pBluescript SK+) (Fermentas, Waltham, MA, USA), phosphate-buffered saline (PBS), 2,2′ -azobis (2-amidinopropane hydrochloride) (AAPH) solution (95 mM in PBS), and the aqueous phase diluted at various concentrations were added to test tubes, to a final volume of 10 μL.

Techniques: Plasmid Preparation

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of the Antioxidant Properties and Bioactivity of Koroneiki and Athinolia Olive Varieties Using In Vitro Cell-Free and Cell-Based Assays

doi: 10.3390/ijms26020743

Figure Lengend Snippet: Heat map of the antioxidant score, corresponding to the antioxidant potential of Greek olive samples for the assays DPPH•, ABTS •+ , Superoxide radical (O 2 •− ), Hydroxyl radical (OH•), Reducing power (RP), and Peroxyl radical (ROO•)-induced plasmid DNA relaxation assay, with respect to different irrigation regimes. The color scale from grey to white represents the Antioxidant score values of the normalized antioxidant capacity data, from low to high, respectively, with 0 representing the lowest antioxidant score and 1 the highest.

Article Snippet: In brief, plasmid DNA (3.2 μg/mL, pBluescript SK+) (Fermentas, Waltham, MA, USA), phosphate-buffered saline (PBS), 2,2′ -azobis (2-amidinopropane hydrochloride) (AAPH) solution (95 mM in PBS), and the aqueous phase diluted at various concentrations were added to test tubes, to a final volume of 10 μL.

Techniques: Plasmid Preparation

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of the Antioxidant Properties and Bioactivity of Koroneiki and Athinolia Olive Varieties Using In Vitro Cell-Free and Cell-Based Assays

doi: 10.3390/ijms26020743

Figure Lengend Snippet: Heat map of the antioxidant score, corresponding to the antioxidant potential of Greek olive samples for the assays DPPH•, ABTS •+ , Superoxide radical (O 2 •− ), Hydroxyl radical (OH•), Reducing power (RP), and Peroxyl radical (ROO•)-induced plasmid DNA relaxation assay, with respect to the altitude. The color scale from gray to white represents the Antioxidant score values of the normalized antioxidant capacity data, from low to high, respectively, with 0 representing the lowest antioxidant score and 1 the highest.

Article Snippet: In brief, plasmid DNA (3.2 μg/mL, pBluescript SK+) (Fermentas, Waltham, MA, USA), phosphate-buffered saline (PBS), 2,2′ -azobis (2-amidinopropane hydrochloride) (AAPH) solution (95 mM in PBS), and the aqueous phase diluted at various concentrations were added to test tubes, to a final volume of 10 μL.

Techniques: Plasmid Preparation

Journal: Journal of Ovarian Research

Article Title: The miR-1290/OGN axis in ovarian cancer-associated fibroblasts modulates cancer cell proliferation and invasion

doi: 10.1186/s13048-024-01364-w

Figure Lengend Snippet: Culture with OGN-overexpressing CAFs-derived conditioned medium inhibits ovarian cancer cell aggressiveness ( A – B ) The mRNA expression and protein levels of OGN were examined in CAFs and NFs by qRT-PCR and Immunoblotting, respectively. ( C – D ) SKOV3 and A2780 cells were co-cultured with CAFs- or NFs-derived conditioned medium (CAFs-CM/NFs-CM) and examined for the mRNA expression and protein levels of OGN in SKOV3 and A2780 cells by qRT-PCR and Immunoblotting, respectively. ( E – F ) OGN overexpression was achieved in CAFs by transducing OGN overexpression vector (OGN-OE); OGN overexpression was confirmed by qRT-PCR and Immunoblotting, respectively. Then, SKOV3 and A2780 cells were cultured in control medium (con-CM), CAFs-CM, NF-CM, CAFs (lv-NC)-CM, or CAFs (OGN-OE)-CM, and examined for cell viability by MTT assay ( G ); DNA synthesis capacity by EdU ( H ); cell invasion by Transwell with chambers pre-coated with Matrigel ( I ); the protein levels of E-cadherin, vimentin, p-mTOR, mTOR, p-Akt, and Akt by Immunoblotting ( J ). ** P < 0.01, compared with NFs or CAFs (NC) group; ## P < 0.01 compared with CAFs (NC)-CM group

Article Snippet: Human ovarian cancer cell line SKOV3 (HTB-77) was procured from ATCC (Manassas, VA, USA) and cultured in McCoy’s 5a Medium Modified (30-2007, ATCC) supplemented with 10% FBS.

Techniques: Derivative Assay, Expressing, Quantitative RT-PCR, Western Blot, Cell Culture, Over Expression, Plasmid Preparation, Control, MTT Assay, DNA Synthesis

Journal: Journal of Ovarian Research

Article Title: The miR-1290/OGN axis in ovarian cancer-associated fibroblasts modulates cancer cell proliferation and invasion

doi: 10.1186/s13048-024-01364-w

Figure Lengend Snippet: The miR-1290/OGN axis in CAFs modulates ovarian cancer cell aggressiveness SKOV3 and A2780 cells were cultured in CAFs co-transduced with miR-1290 mimics and OGN-OE and examined for cell viability by MTT ( A ); DNA synthesis capacity by EdU ( B ); cell invasion by Transwell with chambers pre-coated with Matrigel ( C ); the protein levels of E-cadherin, vimentin, p-mTOR, mTOR, p-Akt, and Akt by Immunoblotting ( D ). ** P < 0.01, compared with CAFs (NC + NC mimics)-CM group; ## P < 0.01 compared with CAFs (OGN-OE + miR-1290 mimics)-CM group

Article Snippet: Human ovarian cancer cell line SKOV3 (HTB-77) was procured from ATCC (Manassas, VA, USA) and cultured in McCoy’s 5a Medium Modified (30-2007, ATCC) supplemented with 10% FBS.

Techniques: Cell Culture, Transduction, DNA Synthesis, Western Blot

Journal: Journal of Ovarian Research

Article Title: The miR-1290/OGN axis in ovarian cancer-associated fibroblasts modulates cancer cell proliferation and invasion

doi: 10.1186/s13048-024-01364-w

Figure Lengend Snippet: Effects of miR-1290-overexpressing or -inhibited CAFs-derived conditioned medium on ovarian cancer cell aggressiveness SKOV3 and A2780 cells were cultured in CAFs transduced with miR-1290 mimics or inhibitor and examined for cell viability by MTT ( A ); DNA synthesis capacity by EdU ( B ); cell invasion by Transwell with chambers pre-coated with Matrigel ( C ); the protein levels of E-cadherin, vimentin, p-mTOR, mTOR, p-Akt, and Akt by Immunoblotting ( D ). ** P < 0.01, compared with CAFs (NC mimics)-CM group; ## P < 0.01 compared with CAFs (NC inhibitor)-CM group

Article Snippet: Human ovarian cancer cell line SKOV3 (HTB-77) was procured from ATCC (Manassas, VA, USA) and cultured in McCoy’s 5a Medium Modified (30-2007, ATCC) supplemented with 10% FBS.

Techniques: Derivative Assay, Cell Culture, Transduction, DNA Synthesis, Western Blot

Journal: Journal of Ovarian Research

Article Title: The miR-1290/OGN axis in ovarian cancer-associated fibroblasts modulates cancer cell proliferation and invasion

doi: 10.1186/s13048-024-01364-w

Figure Lengend Snippet: The miR-1290/OGN axis in CAFs modulates tumorigenic capacity of ovarian cancer cell in vivo Xenograft transplanted tumor models were established in nude mice by injecting a mixture of SKOV3 cells with CAFs or infected CAFs (infected with miR-1290 mimics or lv-OGN) and nude mice were separated into five groups ( n = 6 per group): the SKOV3 cells + CAFs group, the SKOV3 cells + CAFs (lv-NC + NC mimics) group, the SKOV3 cells + CAFs (lv-OGN + NC mimics) group, the SKOV3 cells + CAFs (lv-NC + miR-1290 mimics) group, the SKOV3 cells + CAFs (lv-OGN + miR-1290 mimics) group. ( A ) Images of the tumors in each group. ( B ) Tumor volumes were measured every five days from the 10th day of the experiment. ( C ) Tumor weight were determined at the 30th day. ( D – E ) The mRNA levels of OGN and miR-1290 in mice tumor tissues were detected by qRT-PCR. ( F ) Ki67 and Vimentin levels in tumor tissues were examined using IHC staining. Scale bar = 20 μm. ( G ) The protein levels of E-cadherin, vimentin, p-mTOR, mTOR, p-Akt, and Akt in tumor tissues were determined using immunoblotting. * P < 0.05, ** P < 0.01, compared between SKOV3 cells + CAFs group and SKOV3 cells + CAFs (lv-NC + NC mimics); ## P < 0.01 compared with SKOV3 cells + CAFs (lv-NC + NC mimics) group; && P < 0.01 compared with SKOV3 cells + CAFs (lv-OGN + miR-1290 mimics) group

Article Snippet: Human ovarian cancer cell line SKOV3 (HTB-77) was procured from ATCC (Manassas, VA, USA) and cultured in McCoy’s 5a Medium Modified (30-2007, ATCC) supplemented with 10% FBS.

Techniques: In Vivo, Infection, Quantitative RT-PCR, Immunohistochemistry, Western Blot

Journal: Journal of Biological Chemistry

Article Title: The Estrogen-responsive B Box Protein Is a Novel Regulator of the Retinoid Signal

doi: 10.1074/jbc.m600879200

Figure Lengend Snippet: FIGURE 6. Overexpressed EBBP enhances RAR transcription and induced cell proliferation in retinoid-resistant lung and breast cancer cells. A, induction of RAR gene expression in SK-MES-1, MDA-MB-231, and MDA-MB-468 cancer cells transiently transfected with the full-length human EBBP cDNA or empty vector by quantitative real time PCR. B, inhibition of DNA synthesis in SK-MES-1, MDA-MB-231, and MDA-MB-468 cells after transient transfection with EBBP cDNA and 10 M aRA treatment. *, a statistically significant difference (p 0.05) compared with empty vector (EV) control. **, a statistically significant difference (p 0.01) compared with empty vector control.

Article Snippet: The human lung cancer cell line (SK-MES-1) and breast cancer cells (MDA-MB-231 and MDA-MB468) were obtained from the American Type Culture Collection.

Techniques: Gene Expression, Transfection, Plasmid Preparation, Real-time Polymerase Chain Reaction, Inhibition, DNA Synthesis, Control